Ingredients for culture media

Culture media must be formulated to provide an adequate balance of nutrients in both qualitative and quantitative terms, for optimal growth of microorganisms.

Microorganisms need sources of energy and carbon, such as sugars or amino acids, which change depending on the microorganism sought, for example some are capable of breaking down complex molecules such as starches or cellulose. Peptones provide some of these components, but simple sugars such as glucose or lactose generally need to be added.

As a source of nitrogen, microorganisms can use amino acids or more complex proteins, which are found in peptones and in plant or meat or casein extracts.

Macronutrients such as phosphorus, sulphur, calcium, magnesium, iron, essential amino acids, etc., which are added in the form of salts, vitamins or blood, are also important.

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