To set up the test, it is necessary to have instrumentation for the LAL test, preferably dedicated to it.
One of the cornerstones of the LAL test is the accurate mixing of the endotoxin standard stock solution and the subsequent serial dilutions. The successful outcome of the test also depends on this.
For the gel-clot method, a dry incubator is required, and the reaction mixture must be incubated at 37°C for 60 minutes. The incubator should be checked with a calibrated thermometer to verify that it can maintain the optimal temperature range for the duration of the test.
The kinetic LAL test requires an optical reader for tubes or microplates, which also functions as an incubator and includes dedicated software.
For the precise dispensing of LAL reagent and samples.
For verifying the performance of the dry incubator.
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