Method for the detection of Legionella in environmental samples

Responsible for the majority of cases is Legionella pneumophila, and in particular serogroup 1.

Since this is a risk class 2 microorganism, it is necessary that the samples are taken by expert personnel, equipped with appropriate personal protective equipment and working in laboratories adequately equipped with class II Biohazard hoods, maintaining conditions of environmental sterility and avoiding cross-fertilization. -contamination with any other samples.

Regulations

ISO 11731:2017 Water quality — Enumeration of Legionella

ANALYSIS PROCEDURE ISO 11731:2017

The matrices affected are all types of environmental water samples, including drinking, industrial and natural waters, and associated matrices (sediments, deposits and sludges). The procedure consists of these main phases:

1. Sample collection, transport and storage

2. Concentration and decontamination of the sample

3. Growth on selective culture media

4. Examination of plates after incubation

5. Confirmatory test

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