LAL TEST: GEL CLOT METHOD Technical Summary n. 1

Protocol and materials for performing the LAL test on water, including an in-depth look at the Gel Clot method, performed with multiple and single tests. This section covers all the products available in the Kairosafe catalog for performing the LAL test.

Introduction and bases of LAL test

Focus on the Gel Clot method, a semiquantitative method that allows the presence of endotoxins to be identified through the formation of a stable gel on the bottom of the test tube thanks to the interaction between the lysate and any endotoxins present in the sample.

Interferences

A brief overview of the main sources of interference. For further information, please see the technical guide INTERFERENCE IN THE LAL TEST: SOLUTIONS.

Materials

Overview of LAL TEST REAGENTS, PYROGENIC CONSUMABLES and LAL TEST INSTRUMENTS.

Protocol and results interpretation

What are the main steps of the Gel Clot test, including reagent reconstitution, lysate validation, and the actual test on the sample?

Differences in the LAL test protocol: multiple tests versus single tests.

Test acceptance criteria and result interpretation.

Products for LAL test, reagents, consumables and instruments

The Technical Summary contains a detailed list of reagents, endotoxins, dedicated instrumentation, and pyrogen-free consumables, all necessary for setting up and performing the LAL test using the Gel Clot method.