The LAL test reagent is presented as a fine lyophilized powder, in a vial to be reconstituted with LAL water immediately before use. The reconstituted reagent is aliquoted and mixed with the samples and Standard Endotoxin controls according to the protocols of the chosen method.
Derived from Limulus polyphemus Amebocyte Lysate (LAL), it reacts with endotoxins present in the membranes of Gram-negative bacteria, triggering an enzymatic cascade that leads to coagulation.
With the LAL reagent, the test can be performed using the following methods:
The recombinant LAL reagent represents a sustainable and innovative future for the bacterial endotoxin test. It provides the same reliable results as the conventional LAL reagent without containing animal-derived components, utilizing the same enzymatic cascade and avoiding cross-reactivity with 1,3-β-D-glucans.
The recombinant test is a quantitative kinetic-chromogenic method.
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