qPCR Kit for the Detection of a Specific Pathogen. The test is based on real-time PCR reactions with 5′ nuclease activity to amplify a unique genomic sequence in the target microorganism.
PCR is a method used to amplify a specific DNA sequence in a reaction containing, among other components, a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence.
The DNA molecule denatures when the solution is heated, separating into two strands. Upon cooling, the primers bind to the target sequences on the separated DNA strands, and DNA polymerase synthesizes a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons).
Repeated cycles of denaturation, annealing, and extension exponentially increase the number of target amplicons. In real-time PCR, the signal is measured at each cycle, typically using specific fluorescent probes. Fluorescence is detected by a reader, and the associated software plots fluorescence intensity against the number of cycles, allowing determination of the presence or absence of the target organism.
